| 1. | Detection of lily mottle virus by rt - pcr using special primers and degenerate primers
应用特异引物和简并引物检测百合斑驳病毒 |
| 2. | Automated programming of degenerate primers and the cloning of the diamondback esterase gene
程序化设计简并引物与克隆小菜蛾酯酶基因 |
| 3. | Depending on the conserved amino acids of glycine betaine abc transporter , a pair of degenerate primers were designed
在h . trueperi发现有甘氨酸甜菜碱abc转运系统。 |
| 4. | A pair of degenerate primers were designed as 5 " - gci ytn car gar tty aga t - 3 " / 5 " - cat iac ncc cca ncc - 3 " , and a 630bp - long cdna fragment of d . salina was obtained , which had high similarity to the enolases from other species
对眼藻进行了不同的渗透震动:从1 . 5mnaci到0 . 5mnaci的低渗震动和从1 . 5mnaci到3 . 0mnaci的高渗震动。 |
| 5. | 2 . using a pair of degenerate primers based on the conserved region , hmg - box , of human sry gene , eight different fragments were amplified from both female and male rana rugulosa wiegmann then cloned by using pmd18 - t vector and sequenced
2 、参照人sox基因设计了一对兼并引物，扩增了虎纹蛙的sox基因，并对扩增产物进行了克隆和测序。 |
| 6. | With a pair of degenerate primers designed according to the conserved region among the known cdna sequences of five rodents , a cdna fragment was generated by rt - pcr from total rna isolated from ovaries of lagurus lagurus
首先根据已发表的五种啮齿目动物zp3cdna序列同源性分析结果设计特异性引物， rt - pcr扩增草原兔尾鼠zp3 （ lzp3 ） cdna保守序列。 |
| 7. | Two degenerate primers were designed and synthesized according to the highly conservative sequences among the known - glc genes . a cdna fragment of 208bp was amplified by rt - pcr , which was subsequently cloned into pmd18 - t vector for sequencing analysis
利用genbank中已经登录的其它植物中该酶基因的保守序列，设计一对简并引物，采用rt - pcr技术，从茶树扩增出208bp的cdna片段。 |
| 8. | Depending on the degenerate primers which were designed according to the conserved amino acids of glycine betaine secondary transporter , a fragment about 1 kb was obtained by pcr . the pcr fragment was purified and labeled with dig as a probe
将其总dna用sau3ai部分酶切后，收集4 15kb大小的酶切片段，克隆到用bamhi酶切的puc18中，构建halobacillussp . d8基因组文库，共获得约9000个重组质粒。 |
| 9. | To elucidate further the presense and function of integrin - like protein , we try to clone 3 " terminus seqence of integrin - like gene from germinated pollen of lily by 3 " race using degenerate primers corresponding to the conserved cytoplasmic regions of a and 3 subunits
而要进一步从分子水平上证实类整合素的存在以及研究它的功能，必须克隆其基因。根据动物整合素、亚基的胞质域保守序列设计简并引物，利用3 race的方法扩增、亚基的3末端序列。 |
| 10. | Part two : studies ; l . the sox gene of dinodon refozonatum was amplified by using a pair of primers which can amplify the conservative motif ( hmg - box ) of human sry gene . the amplification band was observed in both male and female dinodon refozonatum , whose length was consistent with that of human sry gene , which about 220bp . the result of sscp analysis showed that there were many differences in the sox gene sequence between dinodon refozonatum and human , and there was a few differences between male and female dinodon refozonatum . 2 . using a pair of degenerate primers based on the conservative region , hmg - box , of human sry gene , six different fragments were amplified from either female or male dinodon refozonatum , then cloned by using pmd18 - t vector and sequenced
结果显示： ( 1 )赤链蛇基因的扩增片段与人sry基因扩增片断大小相同，为220bp左右； ( 2 )雌、雄赤链蛇sox基因的扩增片段大小虽然与人的相同，但其单链迁移率与人的有较大差异，而且雌雄个体间有明显差异，预示该基因的dna序列在雌雄个体中可能有差异； 2 、参照人sry基因hmg - box保守区的序列，又设计一对兼并引物，扩增了赤链蛇的sox基因，并对扩增产物进行了克隆和测序。 |
