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Chinese translation for "degenerated primers"

简并性引物

Related Translations:
to degenerate:  这样会让人堕落
hyaline degenerated:  透明变性的
degenerate semiconductor:  简并半导体简并型半导体退化半导体
degenerate laser:  简并激光器
degenerated cell:  退化细胞
degenerated form:  退化类型退化型
degenerated electrons:  简并化的电子
degenerate parabola:  退化抛物线
degenerate solution:  退化解
degenerate flow:  退化流动
Example Sentences:
1.Detection of lily mottle virus by rt - pcr using special primers and degenerate primers
应用特异引物和简并引物检测百合斑驳病毒
2.Automated programming of degenerate primers and the cloning of the diamondback esterase gene
程序化设计简并引物与克隆小菜蛾酯酶基因
3.Depending on the conserved amino acids of glycine betaine abc transporter , a pair of degenerate primers were designed
在h . trueperi发现有甘氨酸甜菜碱abc转运系统。
4.A pair of degenerate primers were designed as 5 " - gci ytn car gar tty aga t - 3 " / 5 " - cat iac ncc cca ncc - 3 " , and a 630bp - long cdna fragment of d . salina was obtained , which had high similarity to the enolases from other species
对眼藻进行了不同的渗透震动:从1 . 5mnaci到0 . 5mnaci的低渗震动和从1 . 5mnaci到3 . 0mnaci的高渗震动。
5.2 . using a pair of degenerate primers based on the conserved region , hmg - box , of human sry gene , eight different fragments were amplified from both female and male rana rugulosa wiegmann then cloned by using pmd18 - t vector and sequenced
2 、参照人sox基因设计了一对兼并引物,扩增了虎纹蛙的sox基因,并对扩增产物进行了克隆和测序。
6.With a pair of degenerate primers designed according to the conserved region among the known cdna sequences of five rodents , a cdna fragment was generated by rt - pcr from total rna isolated from ovaries of lagurus lagurus
首先根据已发表的五种啮齿目动物zp3cdna序列同源性分析结果设计特异性引物, rt - pcr扩增草原兔尾鼠zp3 ( lzp3 ) cdna保守序列。
7.Two degenerate primers were designed and synthesized according to the highly conservative sequences among the known - glc genes . a cdna fragment of 208bp was amplified by rt - pcr , which was subsequently cloned into pmd18 - t vector for sequencing analysis
利用genbank中已经登录的其它植物中该酶基因的保守序列,设计一对简并引物,采用rt - pcr技术,从茶树扩增出208bp的cdna片段。
8.Depending on the degenerate primers which were designed according to the conserved amino acids of glycine betaine secondary transporter , a fragment about 1 kb was obtained by pcr . the pcr fragment was purified and labeled with dig as a probe
将其总dna用sau3ai部分酶切后,收集4 15kb大小的酶切片段,克隆到用bamhi酶切的puc18中,构建halobacillussp . d8基因组文库,共获得约9000个重组质粒。
9.To elucidate further the presense and function of integrin - like protein , we try to clone 3 " terminus seqence of integrin - like gene from germinated pollen of lily by 3 " race using degenerate primers corresponding to the conserved cytoplasmic regions of a and 3 subunits
而要进一步从分子水平上证实类整合素的存在以及研究它的功能,必须克隆其基因。根据动物整合素、亚基的胞质域保守序列设计简并引物,利用3 race的方法扩增、亚基的3末端序列。
10.Part two : studies ; l . the sox gene of dinodon refozonatum was amplified by using a pair of primers which can amplify the conservative motif ( hmg - box ) of human sry gene . the amplification band was observed in both male and female dinodon refozonatum , whose length was consistent with that of human sry gene , which about 220bp . the result of sscp analysis showed that there were many differences in the sox gene sequence between dinodon refozonatum and human , and there was a few differences between male and female dinodon refozonatum . 2 . using a pair of degenerate primers based on the conservative region , hmg - box , of human sry gene , six different fragments were amplified from either female or male dinodon refozonatum , then cloned by using pmd18 - t vector and sequenced
结果显示: ( 1 )赤链蛇基因的扩增片段与人sry基因扩增片断大小相同,为220bp左右; ( 2 )雌、雄赤链蛇sox基因的扩增片段大小虽然与人的相同,但其单链迁移率与人的有较大差异,而且雌雄个体间有明显差异,预示该基因的dna序列在雌雄个体中可能有差异; 2 、参照人sry基因hmg - box保守区的序列,又设计一对兼并引物,扩增了赤链蛇的sox基因,并对扩增产物进行了克隆和测序。
Similar Words:
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